Overexpression of l-galactono-1, 4-lactone dehydrogenase (GLDH) in Lanzhou lily (Lilium davidii var. unicolor) via particle bombardment-mediated transformation |
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Authors: | Shouguo Shi Fengwang Ma Yonghong Li Fengjuan Feng Zengzhen Shang |
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Institution: | (1) Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China; |
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Abstract: | In China, the native Lanzhou lily (Lilium davidii var. unicolor) is used as an edible lily and is an important source of ascorbic acid. However, an efficient transformation system has not
been developed for this important crop. Here, we describe the first successful genetic transformation mediated by particle
bombardment in this lily variety. We used pCAMBIA-GLDH, which contains the l
-galactono-1, 4-lactone dehydrogenase gene from apple, a hygromycin phosphotransferase gene as a selectable marker, and an intron-containing β-glucuronidase gene
as a reporter. In all, 35 transgenic lines were selected via GUS assays and PCR; from these, eight were confirmed by Southern
analyses. Transgenic plants were transferred to soil and grown under greenhouse conditions. The levels of reduced ascorbate
and total ascorbate (reduced ascorbate + oxidized ascorbate) in transgenic plants were found to be two to seven times those
in non-transgenic controls. |
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Keywords: | |
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