Isolation of protoplast from aerial mycelia and fruit-body formation from regenerated mycelia in Pleurotus cornucopiae |
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| Authors: | Mizoguchi Masahide Kitano Satoshi Takahashi Wataru Sugawara Fuyuki Tanaka Osamu |
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| Institution: | Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto, Higashinada-ku, Kobe, Japan. |
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| Abstract: | We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities. |
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