High-level production of ethanol during fed-batch ethanol fermentation with a controlled aeration rate and non-sterile glucose powder feeding of Saccharomyces cerevisiae |
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Authors: | Hyeon-Beom Seo Seung Seop Kim Hyeon-Yong Lee Kyung-Hwan Jung |
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Institution: | (1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China; |
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Abstract: | In this study, we utilized a unique strategy for fed-batch fermentation using ethanol-tolerant Saccharomyces cerevisiae to achieve a high-level of ethanol production that could be practically applied on an industrial scale. During this study,
the aeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the production
of ethanol. Additionally, non-sterile glucose powder was fed during fed-batch ethanol fermentation and corn-steep liquor (CSL)
in the medium was used as an organic N-source. When aeration was conducted, the ethanol production and productivity were superior
to that when aeration was not conducted. Specifically, the maximum ethanol production reached approximately 160 g/L, when
the fermentor was aerated at 0.13 vvm. These findings indicate that the use of a much less expensive C-source may enable the
fermentation process to be directed towards the improvement of overall ethanol production and productivity in fermentors that
are aerated at 0.13 vvm. Furthermore, if a repeated fed-batch process in which the withdrawal and fill is conducted prior
to 36 h can be employed, aeration at a rate of 0.33 and/or 0.8 vvm may improve the overall ethanol productivity |
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