Construction of a reference molecular linkage map of globe artichoke (<Emphasis Type="Italic">Cynara cardunculus</Emphasis> var. <Emphasis Type="Italic">scolymus</Emphasis>) |
| |
Authors: | E Portis G Mauromicale R Mauro A Acquadro D Scaglione S Lanteri |
| |
Institution: | (1) DIVAPRA Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095 Grugliasco (Turin), Italy;(2) Dipartimento di Scienze Agronomiche, Agrochimiche e delle Produzioni Animali, sez. Scienze Agronomiche, University of Catania, via Valdisavoia 5, 95123 Catania, Italy; |
| |
Abstract: | The genome organization of globe artichoke (Cynara cardunculus var. scolymus), unlike other species belonging to Asteraceae (=Compositae) family (i.e. sunflower, lettuce and chicory), remains largely
unexplored. The species is highly heterozygous and suffers marked inbreeding depression when forced to self-fertilize. Thus
a two-way pseudo-testcross represents the optimal strategy for linkage analysis. Here, we report linkage maps based on the
progeny of a cross between globe artichoke (C. cardunculus var. scolymus) and cultivated cardoon (C. cardunculus var. altilis). The population was genotyped using a variety of PCR-based marker platforms, resulting in the identification of 708 testcross
markers suitable for map construction. The male map consisted of 177 loci arranged in 17 major linkage groups, spanning 1,015.5 cM,
while female map was built with 326 loci arranged into 20 major linkage groups, spanning 1,486.8 cM. The presence of 84 loci
shared between these maps and those previously developed from a cross within globe artichoke allowed for map alignment and
the definition of 17 homologous linkage groups, corresponding to the haploid number of the species. This will provide a favourable
property for QTL scanning; furthermore, as 25 mapped markers (8%) correspond to coding regions, it has an additional value
as functional map and might represent an important genetic tool for candidate gene studies in globe artichoke. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|