Immunocytochemical detection of Ca2+-dependent subspecies of protein kinase C in mouse embryos before and during compaction |
| |
Authors: | Mami Ohsugi Hideki Yamamura Reiji Semba and Hiroyoshi Hidaka |
| |
Institution: | (1) Department of Anatomy, Mie University School of Medicine, Tsu, 514 Mie, Japan;(2) Research Institute of Environmental Medicine, Nagoya University, Chikusa-ku, 464-01 Nagoya, Japan;(3) Department of Pharmacology, Nagoya University School of Medicine, Showa-ku, 466 Nagoya, Japan |
| |
Abstract: | Summary To confirm the possibility that protein kinase C is involved in compaction of mouse embryos, the presence and distribution pattern of Ca2+-dependent subspecies of this enzyme in mouse embryos, before and during compaction, were examined immunocytochemically with three different monoclonal antibodies. These were MC-1a, MC-2a and MC-3a, which selectively interact with the subspecies of the enzyme known as types I, II and III, respectively. Only when embryos were incubated with MC-3a, was immunofluorescence clearly detected in all cells of embryos before and during compaction. This result demonstrates the presence of type III protein kinase C in embryos before and during compaction and suggests the possibility that the type III enzyme may be involved in compaction. No marked differences were found in the distribution pattern of the type III enzyme between embryos examined before and during compaction. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|