Development of nuclear microsatellite markers for the Japanese conifers Tsuga diversifolia and T. sieboldii (Pinaceae) |
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| Authors: | James RP Worth Jong‐Cheol Yang Seiichi Kanetani Satoshi Kikuchi Shota Sakaguchi Tokuko Ihara‐Udino |
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| Institution: | 1. Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305‐8687, Japan;2. Division of Forest Biodiversity and Herbarium, Korea National Arboretum, Pocheon, Gyeonggi, 487‐821, Korea;3. Kyushu Research Center, Forestry and Forest Products Research Institute, 4‐11‐16 Kurokami, Chuo‐ku, Kumamoto, 860‐0862, Japan;4. Graduate School of Human and Environmental Studies, Kyoto University, Yoshida‐ Nihonmatsu‐cho, Sakyo‐ku, Kyoto, 606‐8501, Japan |
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| Abstract: | Nuclear microsatellite markers were developed for the two Tsuga species native to the Japanese Archipelago, Tsuga diversifolia and T. sieboldii, and a population with genetic affinities to T. diversifolia on Ulleung Island, Korea. Tsuga diversifolia and T. sieboldii are widespread dominant trees of temperate and subalpine forests in Japan but to date no genetic markers have been developed for these species. Fifteen polymorphic loci were developed and characterized, of which 14 are reliably amplified in each taxon. Across both species and the Ulleung Island population, the number of alleles per locus ranged from 3 to 26 (average = 13.93) and observed heterozygosity ranged from 0.005 to 0.935 (average = 0.535). In addition, all 15 loci were successfully amplified in a single accession of the Chinese species, T. chinensis. These markers will be useful for investigating the species’ biogeography, range‐wide genetic diversity, conservation genetic issues and potential for hybridisation. |
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| Keywords: | East Asian Tsuga expressed sequence tag hemlock Japanese endemic conifers next generation sequencing |
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