| 烟曲霉rRNA基因ITS区的克隆测序分析 |
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| 引用本文： | 骆志成,王端礼,李若瑜,李世荫,孔繁荣,李冬梅. 烟曲霉rRNA基因ITS区的克隆测序分析[J]. 菌物学报, 2000, 19(3): 336-341 |
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| 作者姓名： | 骆志成 王端礼 李若瑜 李世荫 孔繁荣 李冬梅 |
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| 作者单位： | 北京医科大学第一医院皮肤科北京 100034 |
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| 基金项目： | 卫生部部属院校临床学科重点项目资助基金 |
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| 摘 要： | 对烟曲霉rRNA基因内转录间区(ITS区)进行了克隆测序,并将之与其他几种常见曲霉的相应序列进行了比较.发现3株烟曲霉的ITSⅠ区完全相同,而其中1株烟曲霉的ITSⅡ区与一条已知相应序列仅有2个碱基的变异.提示烟曲霉rRNA基因的两个ITS区序列均十分保守,而且与黄曲霉、黑曲霉、土曲霉及构巢曲霉的相应序列相比较,均有一定程度的变异.
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| 关 键 词： | 烟曲霉 rRNA基因内转录间区 克隆 测序 |
| 文章编号： | 1007-3515(2000)03-0336-0341 |
| 修稿时间： | 2000-01-10 |
COLONING AND SEQUENCING OF THE ITS IN rDNA GENE OF ASPERGILLUS FUMIGATUS |
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| LUO Zhi-Cheng,WANG Duan-Li, LI Ruo-Yu,LI Shi-Yin, KONG Fan-Rong,LI Deng-Mei. COLONING AND SEQUENCING OF THE ITS IN rDNA GENE OF ASPERGILLUS FUMIGATUS[J]. Mycosystema, 2000, 19(3): 336-341 |
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| Authors: | LUO Zhi-Cheng WANG Duan-Li LI Ruo-Yu LI Shi-Yin KONG Fan-Rong LI Deng-Mei |
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| Abstract: | The internal transcribed spacer (ITS) region in rRNA gene of Aspergillus fumigatus was cloned and sequenced, and the sequences were compared with those of other common Aspergillus spp. The results showed that the 3 isolates of A. fumigatus with different origins have the same ITS I seqdence, while 1 isolate of A.fumigatus has the almost same ITS II sequence in compdrison with that from the GenBank, except for a variance of two bases. It is suggested that the ITS I and ITS II sequences of A.fumigatus both be conserved, and varied in certain extent from those of A.flavus, A. niger, A. terrus and A. nidulans. |
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| Keywords: | Aspergillus fumigatus ITS Cloning Sequencing |
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