Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system |
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| Authors: | M. Jacobs-Lorena C. Baglioni |
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| Affiliation: | (1) Department of Biology, Massachusetts Institute of Technology, 02139 Cambridge, Mass., USA;(2) Laboratory of Cell Biology, Rome, Italy |
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| Abstract: | Formyl-[35S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[35S]met-tRNAf from yeast. Most of the [35S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system. |
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