Chemo-enzymatic synthesis of poly-<Emphasis Type="Italic">N</Emphasis>-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces |
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Authors: | Birgit Sauerzapfe Karel K?enek Judith Schmiedel Warren W Wakarchuk Helena Pelantová Vladimir K?en Lothar Elling |
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Institution: | (1) Laboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany;(2) Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic;(3) Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, K1A 0R6, Canada |
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Abstract: | Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion
to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures
and subsequent binding of ECM glycoproteins. First, we synthesized β-GlcNAc glycosides carrying a linker for controlled coupling
onto chemically functionalized surfaces. Then we produced poly-LacNAc structures with defined lengths using human β1,4-galactosyltransferase-1
and β1,3-N-acetylglucosaminyltransferase from Helicobacter pylori. These compounds were also used for kinetic characterization of glycosyltransferases and lectin binding assays. A mixture
of poly-LacNAc-structures covalently coupled to functionalized microtiter plates were identified for best binding to our model
galectin His6CGL2. We further demonstrate for the first time that these poly-LacNAc surfaces are suitable for further galectin-mediated
binding of the ECM glycoproteins laminin and fibronectin. This new technology should facilitate cell adhesion to biofunctionalized
surfaces by imitating the natural ECM microenvironment.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Poly-LacNAc Chemo-enzymatic synthesis Galectin binding ECM glycoproteins Biomaterials |
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