| 我国产日本医蛭水蛭素的分离和纯化 |
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| 引用本文： | 杨潼,李建秋.我国产日本医蛭水蛭素的分离和纯化[J].水生生物学报,1997,21(2):169-173. |
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| 作者姓名： | 杨潼 李建秋 |
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| 作者单位： | 中国科学院水生生物研究所!武汉，430072，中国科学院水生生物研究所!武汉，430072，武汉大学生物化学系!430072 |
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| 摘 要： | 采用离子交换柱层析与凝胶过滤柱层析的方法对从我国产的日本医蛙［Hirudonipponica（whitman）］活体中提取出的唾液腺分泌物进行分离和纯化，获得了较纯净的水蛭素（Hirudin）。测定结果表明：日本医蛭唾液腺分泌物提取液中水蛭素含量为4AT－U／ml；纯化总得率为15％；纯化后的产物比活力为6708AT－U／mg蛋白。
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| 关 键 词： | 水蛭素 阴离子交换柱层析 凝胶过滤柱层析 凝血酶滴定法 |
ISOLATION AND PURIFICATION OF HIRUDIN FROM A BLOOD-SUCKING LEECH,HIRUDO NIPPOIA,IN CHINA |
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| Yang Tong, Li Jianqiu, Yin Ping.ISOLATION AND PURIFICATION OF HIRUDIN FROM A BLOOD-SUCKING LEECH,HIRUDO NIPPOIA,IN CHINA[J].Acta Hydrobiologica Sinica,1997,21(2):169-173. |
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| Authors: | Yang Tong Li Jianqiu Yin Ping |
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| Abstract: | This paper deals with the isolation and purification of hirudin from a bloodsucking leech, Hirudo nipponia in China. Essentially, the experimental process requires an anion exchange chromatography on DEAE-C(52) and a gel filtration chromatography on Sephadex-G(25). The hirudin activity were measured by the method of titration with thrombin. The salivary gland secretions have been obtained from the alive leeches. The hirudin content in salivary gland secretions is 4 AT-U/ml;the hirudin production rate is 15%; ratio activity after purifing is 6708 AT-U/ml protein. |
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| Keywords: | Hirudin Anion exchange chromatography Gel filtration chromatography Method of titration with thrombin |
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