Simplified elimination of rabbit anti-rat acute phase protein IgG that shows cross reactivity with albumin |
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| Authors: | In Gyu Kim Hye Won Shim Kang Suk Lee |
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| Affiliation: | (1) Department of Radiation Biology, Environmental Radiation Research group, Korea Atomic Energy Research Institute, P.O. Box 105, Yusong Taejon, Korea |
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| Abstract: | Antibody preparations against rat acute phase proteins were tested for cross reactivity with other serum proteins, including rat albumin. Rabbit anti-rat a alpha1-acid glycoprotein and ceruloplasmin IgG purified on protein A-Sepharose did not show any cross reactivity with rat albumin, hemoglobin or transferrin. Rabbit anti-rat haptoglobin and -macroglobulin IgG purified on protein A-Sepharose showed a 39% and 30% cross reactivity with rat albumin and a 20% and 19% cross reactivity with rat hemoglobin. Because these proteins in whole serum were not adsorbed on Cibacron Blue F3-GA Sepharose, the albumin would be adsorbed on Cibacron Blue F3-GA Sepharose by the use of whole rat serum. Rabbit anti-rat haptoglobin and alpha2-macroglobulin IgG showing cross reactivity with albumin was simply eliminated. |
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