Molecular cloning of the cDNA encoding a stress-inducible protein phosphatase 1 (PP1) catalytic subunit from French bean (Phaseolus vulgaris L.) |
| |
Authors: | Alfred Zimmerlin Steve C Jupe G Paul Bolwell |
| |
Institution: | (1) Dept of Biochemistry, Royal Holloway and Bedford New College, University of London, TW20 OEX Egham, Survey, UK;(2) Present address: Département de Biologie Moléculaire des Plantes, CNRS-IBMP, 28 rue Goethe, 67083 Strasbourg, France |
| |
Abstract: | A cDNA showing high sequence similarity (>70%) to plant protein phosphatase 1 catalytic subunit variants from other species has been isolated from a cDNA library derived from mRNAs expressed in elicitor-treated suspension-cultured cells. The clone appears to be a near full-length 1431 bp with a 172 bp 5 -untranslated region and a 317 bp 3 -untranslated region. The open reading frame, determined by sequence similarity, codes for a protein with predicted M
r
of 35552. Alternatively an ATG situated to the 5 end of the putative start site would increase the protein size by 6 amino acids.The mRNA for Pvpp1 was shown to be rapidly induced by elicitor treatment of suspension-cultured cells of French bean. The cloned cDNA represents one of the few examples of a gene product that is probably involved in dephosphorylation events arising after the initial responses to biotic stress.Abbreviations PAL
phenylalanine ammonia-lyase
- PP1
protein phosphatase 1
- Pvpp1
Phaseolus vulgaris protein phosphatase 1 |
| |
Keywords: | French bean Phaseolus vulgaris L elicitor stress protein phosphatase induction |
本文献已被 SpringerLink 等数据库收录! |
|