Two-dimensional gel electrophoresis of cattle plasma proteins: Genetic polymorphism of an α1-protease inhibitor |
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Authors: | R. K. Juneja B. Gahne |
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Affiliation: | Department of Animal Breeding and Genetics, The Swedish University of Agricultural Sciences, S-750 07 Uppsala 7, Sweden |
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Abstract: | Two-dimensional electrophoretic analysis of cattle plasma proteins was done by a first dimension separation in agarose gel (pH 5.0), followed by a second dimension in horizontal polyacrylamide gel (pH 9.0). This method resulted in improved and reproducible separation of many α- and β-globulins. Three groups of a-globulins, designated Pi-1, Pi-2 and Pi-3, were found to inhibit the estero-lytic activity of bovine trypsin and bovine chymotrypsin. Pi-2 showed appreciable inhibition only for trypsin and genetic polymorphism was observed for this protein. Family data supported the hypothesis that the three Pi-2 types observed were controlled by two codominant, autosomal alleles. The occurrence of a third Pi-2 allele was also postulated in some animals studied. The frequency of the most common allele, Pi-2s, ranged from 0.5-0.8 in the different breeds of cattle studied (Swedish Red and White, Friesian, Jersey, Charolais and Simmental). The post-transferrins Ptf-1 and Ptf-2 in cattle plasma were shown to be two different genetic systems. |
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Keywords: | Cattle plasma α1,-protease inhibitors α1-antitrypsins genetic polymorphism gene frequency two-dimensional gel electrophoresis |
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