Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of <Emphasis Type="Italic">Catharanthus roseus</Emphasis> |
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Authors: | Murugesan Dhandapani Doo Hwan Kim Seung-Beom Hong |
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Institution: | (1) Department of Molecular Biotechnology, Konkuk University, Seoul, 143-701, South Korea;(2) Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea |
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Abstract: | High-frequency plant regeneration of C. roseus cv. ‘little bright eye’ via somatic embryogenesis and organogenesis from five out of six explants was standardized. Two factors
were found to be important for regeneration: (1) the type of explants, and (2) the combination and concentrations of plant
growth regulators. The highest regeneration percentage through somatic embryogenesis was obtained from mature zygotic embryo
in MS medium supplemented with 7.5 μM of thidiazuron (TDZ). The mature embryo also regenerated efficiently via organogenesis
in MS medium supplemented with either 2.5 μM TDZ or 5.3 μM α-naphthalene acetic acid (NAA) and 2.2 μM 6-benzylaminopurine
(BA). Hypocotyl and cotyledon did not induce somatic embryogenesis and organogenesis in TDZ-containing medium but gave a maximum
percentage of shoots in MS medium supplemented with 5.3 μM NAA and 2.2 μM BA. Stem nodes and meristem tips showed better regeneration
via organogenesis in the medium supplemented with NAA and BA and in lower concentrations of TDZ. |
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Keywords: | Catharanthus roseus Mature embryo Hypocotyl Stem node Shoot tip Somatic embryo Plant regeneration |
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