Loop-driven conformational transition between the alternative and collapsed form of prethrombin-2: targeted molecular dynamics study |
| |
Authors: | Sangwook Wu |
| |
Institution: | Department of Physics, Pukyong National University, Busan 608-737, Republic of Korea |
| |
Abstract: | Two distinct crystal structures of prethrombin-2, the alternative and collapsed forms, are elucidated by X-ray crystallogrphy. We analyzed the conformational transition from the alternative to the collapsed form employing targeted molecular dynamics (TMD) simulation. Despite small RMSD difference in the two X-ray crystal structures, some hydrophobic residues (W60d, W148, W215, and F227) show a significant difference between the two conformations. TMD simulation shows that the four hydrophobic residues undergo concerted movement from dimer to trimer transition via tetramer state in the conformational change from the alternative to the collapsed form. We reveal that the concerted movement of the four hydrophobic residues is controlled by movement of specific loop regions behind. In this paper, we propose a sequential scenario for the conformational transition from the alternative form to the collapsed form, which is partially supported by the mutant W148A simulation. |
| |
Keywords: | prethrombin-2 hydrophobic cluster collapsed form alternative form targeted molecular dynamics |
|
|