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Expression of Chlamydia trachomatis inclusion membrane protein genes IncB and IncC in Escherichia coli
Authors:E. S. Kostrjukova  V. N. Lazarev  G. A. Titova  T. A. Akopian  S. A. Levitskii  V. M. Govorun
Affiliation:(1) Research Institute for Physico-Chemical Medicine, Ministry of Health and Social Development of the Russian Federation, ul. Malaya Pirogovskaya 1a, 119992 Moscow, Russia
Abstract:In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins. As a result, we have obtained the first full-length recombinant C. trachomatis proteins IncB and IncC, which can be used for following antibody production and for study of their protein-protein interaction.
Keywords:Chlamydia trachomatis  inclusion membrane protein  gene polymorphism  gene expression
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