Expression of Chlamydia trachomatis inclusion membrane protein genes IncB and IncC in Escherichia coli |
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Authors: | E. S. Kostrjukova V. N. Lazarev G. A. Titova T. A. Akopian S. A. Levitskii V. M. Govorun |
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Affiliation: | (1) Research Institute for Physico-Chemical Medicine, Ministry of Health and Social Development of the Russian Federation, ul. Malaya Pirogovskaya 1a, 119992 Moscow, Russia |
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Abstract: | In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins. As a result, we have obtained the first full-length recombinant C. trachomatis proteins IncB and IncC, which can be used for following antibody production and for study of their protein-protein interaction. |
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Keywords: | Chlamydia trachomatis inclusion membrane protein gene polymorphism gene expression |
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