Activated oxygen species and two extracellular enzymes: laccase and aryl-alcohol oxidase, novel for the lignin-degrading fungus Fusarium proliferatum |
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| Authors: | V. Regalado F. Perestelo A. Rodríguez A. Carnicero F. J. Sosa G. De la Fuente M. A. Falcón |
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| Affiliation: | (1) Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain e-mail: mafalcon@ull.es Tel.: +34-922-318479 Fax: +34-922-630095, ES;(2) Instituto de Productos Naturales y Agrobiología del CSIC, 38206 La Laguna, Tenerife, Spain, ES |
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| Abstract: | Laccase, aryl-alcohol oxidase and superoxide radicals were detected in ligninolytic cultures of Fusarium proliferatum. Enzyme activities were present during the secondary metabolism and seen as single protein bands after non-denaturing electrophoresis. In contrast, superoxide radicals were detected during primary growth, correlating with maximal lignin mineralization. Moreover, ligninolysis decreased when scavengers of both superoxide and hydroxyl radicals were added to cultures, indicating that activated oxygen species are involved in lignin degradation. Received: 12 October 1998 / Accepted: 24 October 1998 |
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