Proteomic map of <Emphasis Type="Italic">Trypanosoma cruzi</Emphasis> CL Brener: the reference strain of the genome project |
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Authors: | Cátia Lacerda Sodré Alexander D Chapeaurouge Dário Eluan Kalume Leila de Mendonça Lima Jonas Perales Octavio Fernandes |
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Institution: | (1) Laboratory of Molecular Epidemiology of Infectious Diseases, Oswaldo Cruz Institute-IOC, Oswaldo Cruz Foundation-FIOCRUZ. Av. Brasil, 4365-Manguinhos, Rio de Janeiro, RJ, 21045-900, Brazil;(2) Laboratory of Toxicology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil;(3) Laboratory for Functional Genomics and Bioinformatics, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil |
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Abstract: | In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction
of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at
4–7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock
proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian
tissues that is involved in the energy supply of the parasite, were also detected. |
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Keywords: | Proteome Trypanosoma cruzi Epimastigote CL Brener strain |
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