Silence of streptomycin 6-phosphotransferase gene derived by incubation at a high temperature inStreptomyces griseus |
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Authors: | Hidenori Shinkawa Masanori Sugiyama Yuji Hatada Takeshi Ohuchi Masao Udagawa Osamu Nimi |
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Affiliation: | (1) Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Kagamiyama 1-4-1, Higashi-Hiroshima 724, Japan;(2) Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan |
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Abstract: | Summary The bald mutants from streptomycin (SM)-producingStreptomyces griseus 2247 obtained by incubation at high temperature (36° C), designated as HT strains, lost resistance to their own antibiotic and scarcely produced the antibiotic. Although SM susceptibility in the mutant was due to loss of SM 6-phosphotransferase activity produced in the cell, the gene coding for the enzyme cloned from an HT strain was surely expressed inS. lividans 1326 as a host. Northern blot analysis showed that the corresponding RNA is not detected in the mutant, indicating that though the gene encoding SM 6-phosphotransferase, at least, the structural gene is not deleted in the cell, the expression is silent. |
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