| 金荞麦和苦荞麦抗菌活性内生真菌的筛选及鉴定 |
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| 引用本文： | 张传博,陈荣林,殷幼平,王中康.金荞麦和苦荞麦抗菌活性内生真菌的筛选及鉴定[J].微生物学通报,2011,38(1):70-77. |
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| 作者姓名： | 张传博 陈荣林 殷幼平 王中康 |
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| 作者单位： | 1. 贵州师范大学生命科学学院,贵州,贵阳,550001
2. 重庆大学生物工程学院,重庆市杀虫真菌农药工程技术研究中心,重庆,400044 |
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| 基金项目： | 贵州省科技公关项目[黔科合GY字 (2005) 3028号]; 贵州省自然科学基金项目[黔科合J字(2007) 2048号]; 校青年教师科
研发展基金 |
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| 摘 要： | 从药用植物金荞麦和苦荞麦的根、茎、叶、花中分离到62株内生真菌,并以金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subtilis)CMCC(B)63501]、小麦赤霉病菌(Fusarium graminearum)、黄瓜枯萎病菌(Fusarium oxysporum f.sp.cu-cumerinum)和绵腐病菌(Pythium aphanidermatum)6种微生物为指示菌对分离到的内生真菌进行抗菌活性检测。结果发现,分离的内生真菌菌株KQH-01、KQH-02和JQY-1的发酵醇提取物具有较好的抑菌活性。形态学特征和分子鉴定确定菌株KQH-01为炭角菌属(Xylaria sp.)真菌,菌株KQH-02为球毛壳菌(Chaetomium globosum),菌株JQY-1为葡萄座腔菌(Botryosphaeria dothidea)。
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| 关 键 词： | 金荞麦 苦荞麦 内生真菌 抑菌活性 分子鉴定 |
| 收稿时间： | 2010/8/15 0:00:00 |
| 修稿时间： | 2010/11/18 0:00:00 |
Isolation and identification of endophytic fungi with antimicrobial activity from Fagopyrum dibotrys and Fagopyrum tataricum |
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| ZHANG Chuan-Bo,CHEN Rong-Lin,YIN You-Ping and WANG Zhong-Kang.Isolation and identification of endophytic fungi with antimicrobial activity from Fagopyrum dibotrys and Fagopyrum tataricum[J].Microbiology,2011,38(1):70-77. |
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| Authors: | ZHANG Chuan-Bo CHEN Rong-Lin YIN You-Ping and WANG Zhong-Kang |
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| Institution: | Shool of Life Sciences, Guizhou Normal University, Guiyang, Guizhou 550001, China;Chongqing Engineering Research Center for Fungal Insecticides, Bioengineering College of Chongqing University, Chongqing 400044, China;Chongqing Engineering Research Center for Fungal Insecticides, Bioengineering College of Chongqing University, Chongqing 400044, China;Chongqing Engineering Research Center for Fungal Insecticides, Bioengineering College of Chongqing University, Chongqing 400044, China |
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| Abstract: | A total of 62 endophytic fungi were isolated from the plant tissues (root, stem, leaf and flower) of medicinal plants Fagopyrum dibotrys and Fagopyrum tataricum. The antimicrobial activity from isolated endophytic fungi were detected using agar diffusion method with Staphylococcus aureus, Es-cherichia coli, Bacillus subtilis CMCC (B) 63501], Fusarium graminearum, Fusarium oxysporum f. sp. cucumerinum and Pythium aphanidermatum as indicator microorganisms. The results showed that the ethanol extracts of three isolated entophytic fungi strain KQH-01, KQH-02 and JQY-1 had relatively strong antibiotic activity to some indicator microorganisms. By using morphological and molecular identification, strain KQH-01was determined to belong to the genus Xylaria sp., and strains KQH-02 and JQY-1 were identified as Chaetomium globosum and Botryosphaeria dothidea respectively. |
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| Keywords: | Fagopyrum dibotrys Fagopyrum tataricum Endophytic fungi Antimicrobial activity Molecular identifi-cation |
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