Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa |
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| Authors: | Zhao Sheng Zhou Kai Guo Abdelrahman Abdou Elbaz Zhi Min Yang |
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| Institution: | 1. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;2. Co Laboratory of Nanjing Agricultural University and Carl Zeiss Far East, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;1. CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India;2. Department of Botany, Lucknow University, Lucknow 226 007, India;3. Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003-9320, USA;4. Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU, UK;1. Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur Agronomique, Chott-Mariem, Sousse, Tunisia;2. Laboratory of Plant Physiology, Department of Biology, Universidad Autónoma de Madrid, 28049 Madrid, Spain;3. Laboratoire Biochimie et Physiologie Moléculaire des Plantes, UMR5004 (CNRS/INRA/SupAgro/UM2), Montpellier, France;4. Centre Régional des Recherches en Horticulture et Agriculture Biologique Chott-Mariem, Sousse, Tunisia |
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| Abstract: | Salicylic acid (SA) as a signal molecule mediates many biotic and environmental stress-induced physiological responses in plants. In this study, we investigated the role of SA in regulating Hg-induced oxidative stress in the roots of alfalfa (Medicago sativa). Plants pretreated with 0.2 mM SA for 12 h and subsequently exposed to 10 μM Hg2+ for 24 h displayed attenuated toxicity to the root. The SA-promoted root growth was correlated with decreased lipid peroxidation in root cells. The ameliorating effect of SA was confirmed by the histochemical staining for the detection of loss of membrane integrity in Hg-treated roots. We show that treatment with 0.2 mM SA increased the activity of NADH oxidase, ascorbate peroxidase (APX) and peroxidase (POD) in the roots exposed Hg. However, a slightly decreased superoxide dismutase (SOD) activity was observed in SA + Hg-treated roots when compared to those of Hg treatment alone. We also measured accumulation of ascorbate (ASC), glutathione (GSH) and proline in the roots of alfalfa and found that roots treated with SA in the presence of Hg accumulated more ASC, GSH and proline than those treated with Hg only. These results suggest that exogenous SA may improve the tolerance of the plant to the Hg toxicity. |
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