Sensitive determination of estriol-16-glucuronide using surface plasmon resonance sensing |
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| Authors: | Xiuqian Jiang Mark Waterland Len Blackwell Yinqiu Wu Krishanthi P Jayasundera Ashton Partridge |
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| Institution: | 1. Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand;2. Biosensors and Biomeasurement, The New Zealand Institute for Plant and Food Research Limited, Private Bag 3123, Waikato Mail Centre, Hamilton, New Zealand Zealand;1. Department of Surgery, Medical College of Wisconsin, Milwaukee, WI;2. Department of Radiology, Medical College of Wisconsin, Milwaukee, WI;1. Department of Anesthesiology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, United States;2. Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, United States;3. Department of Biomedical Sciences, Long Island University, Brookville, NY 11548, United States;4. Department of Chemical and Biomolecular Engineering, NYU-Polytechnic Institute, Brooklyn, NY 11201, United States;1. Canadian Rivers Institute, Department of Biology, University of Prince Edward Island, Charlottetown, Canada;2. Friedrich Loeffler Institute, Insel Reims, Germany;3. Department of Animal and Poultry Science and the Toxicology Centre, University of Saskatchewan, Saskatoon, Canada;1. Serviço de Endocrinologia, Diabetes e Metabolismo, Centro Hospitalar de São João, Porto, Portugal;2. Faculdade de Medicina da Universidade do Porto, Porto, Portugal;3. Serviço de Cirurgia Geral, Centro Hospitalar de São João, Porto, Portugal;4. Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal;1. Centro de Estudos do Mar, Universidade Federal do Paraná, Caixa Postal 61, 83255-976 Pontal do Paraná, PR, Brazil;2. Departamento de Geoquímica, Universidade Federal Fluminense, Morro do Valonguinho s/n, Niterói, Rio de Janeiro, RJ, Brazil;3. Programa de Pós-Graduação em Sistemas Costeiros e Oceânicos (PGSISCO) da Universidade Federal do Paraná, Caixa Postal 61, 83255-976 Pontal do Paraná, PR, Brazil;4. Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-120 São Paulo, SP, Brazil;1. Research Institute of Pulmonology, St. Petersburg State I. Pavlov Medical University, 197089 St. Petersburg, Russia Federation;2. Department of Clinical and Laboratory Diagnostics, St. Petersburg State I. Pavlov Medical University, L.Tolstoy St., 6/8, 197089 St. Petersburg, Russia Federation;3. Pasteur Research Institute of Epidemiology and Microbiology, 197101 St. Petersburg, Russia Federation |
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| Abstract: | For the quantitative evaluation of low levels of an estriol metabolite of estriol (estriol-16-glucuronide (E3-16G)) in liquid media, we developed a simple and highly sensitive immunoassay using a surface plasmon resonance (SPR) biosensor which did not require any time-consuming sample pretreatment steps. E3-16G was conjugated to ovalbumin (OVA) through an oligoethylene glycol (OEG) linker to form protein conjugates (E3-16G-OEG-OVA), which were then immobilized on a carboxymethyl dextran-coated sensor chip via amine coupling to develop inhibition immunoassays. A limit of detection (LOD) of 76 pg/mL was achieved using a rabbit anti-sheep primary antibody as a binding agent. The detection limit was further improved by using synthesized gold colloids (15 nm) as high mass labels conjugated to the primary antibody. In this Au nanoparticle-enhanced assay, the concentration of E3-16G in aqueous samples could be determined in 7.5 min at a level as low as 14 pg/mL. In addition, the high stability of the E3-16G-OEG-OVA surface gave no obvious drop in antibody-binding capability after more than 1000 binding/regeneration cycles which significantly lowered the research cost. |
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