Expression of the Arabidopsis metallothionein 2b enhances arsenite sensitivity and root to shoot translocation in tobacco |
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| Authors: | Veerle M.J. Grispen Barbara Irtelli Henk W.J. Hakvoort Riet Vooijs Tijs Bliek Wilma M. ten Bookum Jos A.C. Verkleij Henk Schat |
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| Affiliation: | 1. Institute of Molecular Cell Biology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands;2. Dipartimento di Chimica e Biotecnologie Agrarie, Università di Pisa, Via del Borghetto, 80, 56124 Pisa, Italy;1. Katedra i Zak?ad Fizjologii Pomorskiego Uniwersytetu Medycznego, Kierownik: prof. dr hab. n. med. Mariusz Z. Ratajczak, Szczecin, Polska;2. Stem Cell Institute at James Graham Brown Cancer Center University of Louisville, Louisville, Kentucky, USA;1. Department of Characterization, Quality and Security, Institute of Food Science Technology and Nutrition (ICTAN-CSIC), Jose Antonio Novais 10, Madrid 28040, Spain;2. Unit Service of Analytical Techniques, Instrumentation and Microbiology (USTA), Institute of Food Science Technology and Nutrition (ICTAN-CSIC), Madrid, Spain;1. Department of Health Sciences, University of Milano-Bicocca, via Cadore 48, 20900 Monza, MI, Italy;2. Department of Pharmacological and Biomolecular Sciences, University of Milan, via Trentacoste 2, 20134 Milan, Italy;1. Department of Chemistry, Capital Normal University, Beijing 100048, PR China;2. Beijing Key Laboratory of Radioactive Waste Treatment, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, PR China;3. Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, PR China;1. Key Laboratory of Soil Environment & Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland 4072, Australia;1. Ito Glycotrilogy Project, ERATO, Japan Science and Technology Agency (JST), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;2. Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;3. Institute of Glycoscience, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan |
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| Abstract: | We expressed the AtMT2b gene under the 35 S cauliflower mosaic virus promoter in Nicotiana tabacum (Sr1), using leaf disc transformation. Arsenite tolerance and uptake, as well as arsenite-induced phytochelatin (PC) accumulation in roots were measured in transgenic lines, and compared to untransformed (‘wild type’) tobacco. Measured after 5 days of exposure, arsenite tolerance was slightly but significantly decreased in the transgenic lines compared to wild type. The highest AtMT2b expressing line exhibited a significantly decreased arsenic accumulation in roots, but an increased accumulation in shoots, while the total amount of arsenic taken up remained unchanged, suggesting that AtMT2b expression enhanced the arsenic root to shoot transport. The same transformant line also exhibited a decreased rate of phytochelatin accumulation in the roots, but the phytochelatin-SH to As molar ratio was higher than in wild type, suggesting that the lower arsenite tolerance in the transformant lines was not due to a potential shortage of cysteine for PC synthesis, imposed by expression of the transgene. |
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