Methyl jasmonate-induced antioxidant defence in root apoplast from sunflower seedlings |
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| Authors: | Maria Carmen Parra-Lobato Nieves Fernandez-Garcia Enrique Olmos Maria Carmen Alvarez-Tinaut Maria Carmen Gómez-Jiménez |
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| Institution: | 1. Department of Plant Physiology, Faculty of Science, University of Extremadura, 06071-Badajoz, Spain;2. CEBAS-CSIC, Campus de Espinardo, P.O. Box 164, 30100-Murcia, Spain;1. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, PR China;2. Key Laboratory of State Forestry Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming, 650224, PR China;1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China;2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;1. Department of Experimental Biology, Masaryk University, Brno, Czechia;2. Institute of Plant Nutrition and Soil Science, Kiel University, Kiel, Germany;3. Institute of Crop Science, Division of Crop Product Quality, University of Hohenheim, Emil-Wolff-Straße 25, 70599 Stuttgart, Germany;4. Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany Academy of Sciences of the Czech Republic, Olomouc, Czechia;1. Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization), Haikou, 570311, China;2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China;3. Hainan Medical University, Haikou, 570102, China;1. Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University, AR 72467, USA;2. Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA |
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| Abstract: | We have investigated the physiological functions of the rapid generation of reactive oxygen species (ROS) and the implication of the antioxidant enzymes in the apoplast and symplast of roots of sunflower (Helianthus annuus L.) seedlings exposed to methyl jasmonate (MeJA, 50 μM). MeJA-elicited roots showed a fast increase in ROS content, followed by a marked increase in the activity of H2O2-scavenging enzymes, guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and catalase (CAT). The mechanisms responsible for MeJA-induced H2O2 accumulation was investigated further by studying both the production and scavenging of H2O2 in the extracellular matrix. Peroxidases active against (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid], ABTS) and guaiacol were found in the apoplastic fluid, and proved to be ionically and covalently associated with sunflower cell walls, although only the peroxidase activities of the soluble apoplastic fractions and those ionically linked to the cell wall were correlated with the accumulation of the H2O2 detected. The results indicated that H2O2 accumulation is a complex and highly regulated event requiring the time-dependent stimulation and down-regulation of differently located enzymes, some of which are involved in H2O2 generation and degradation. It is concluded that exogenous MeJA may be involved in the oxidative stress processes by regulating antioxidant enzyme activities. |
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