AspC基因导入前后E.coli BL21蛋白质组的解析

为了考察表达天冬氨酸转氨酶工程菌在转基因前后蛋白质水平的差异变化，采用固相pH梯度-SDS聚丙烯酰胺双向凝胶电泳对转基因前后的大肠杆菌（E．coli BL21）的总蛋白进行分离，银染、显色后，使用2D蛋白质图象分析系统Image Master 2D Platinum 5．0和SWISS-2D PAGE蛋白质组数据库对双向电泳图谱进行分析，识别了近600个蛋白点，比较分析了与苯丙氨酸合成途径相关的关键蛋白的差异，初步探讨了AspC基因的导入后大肠杆菌蛋白质水平的精细调控。

Comparative proteome analysis of AspC gene overexpression in E.coli BL21

To investigation the difference of proteome level of the recombinant E.coli strain expressing(Aspartate) aminotransferase(AspAT) before and after transformation,two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients was used for isolation and the spots of protein were stained with a(protein) silver staining kit.The the gel were scanned and digitized to create computer images.About 600 protein spots were detected by the Image Master 2D Platinum 5.0 and analyzed by the SWISS-2D PAGE proteome(database).Difference of the key proteins correlated to synthesis of L-Phenylalanine was analyzed.Based on the (effect) of AspC gene analyzed,elaborate regulated of proteome level was primarily discussed in the article.