The effect of hydrogen peroxide on spores of Clostridium perfringens |
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Authors: | Y Ando T Tsuzuki |
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Institution: | Hokkaido Institute of Public Health, Sapporo 060, Japan |
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Abstract: | Dithiothreitol (DTT)-treated spores of Clostridium perfringens were much more sensitive to lysis by H2O2 in the presence of Cu2+ than untreated spores. Lysis was greatly inhibited by hydroxyl radical (.OH) scavengers such as thiourea, dimethylthiourea and dimethylsulfoxide, suggesting that lysis of spores by H2O2 involves formation of OH by Cu2+-catalysed decomposition of the peroxide. DTT-treated spores took up Cu2+ at almost the same rate and extent as did isolated cortical fragments. Hydrogen peroxide caused both the decrease in optical density and the hexosamine solubilization of cortical fragments which bound Cu2+. |
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