A strict anaerobic extreme thermophilic hydrogen-producing culture enriched from digested household waste |
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Authors: | D Karakashev S M Kotay E Trably I Angelidaki |
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Institution: | Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, Kongens Lyngby, Denmark; UR050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne, France |
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Abstract: | Aims: The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H2) producers from digested household solid wastes. Methods and Results: A strict anaerobic extreme thermophilic H2 producing bacterial culture was enriched from a lab-scale digester treating household wastes at 70°C. The enriched mixed culture consisted of two rod-shaped bacterial members growing at an optimal temperature of 80°C and an optimal pH 8·1. The culture was able to utilize glucose, galactose, mannose, xylose, arabinose, maltose, sucrose, pyruvate and glycerol as carbon sources. Growth on glucose produced acetate, H2 and carbon dioxide. Maximal H2 production rate on glucose was 1·1 mmol l?1 h?1 with a maximum H2 yield of 1·9 mole H2 per mole glucose. 16S ribosomal DNA clone library analyses showed that the culture members were phylogenetically affiliated to the genera Bacillus and Clostridium. Relative abundance of the culture members, assessed by fluorescence in situ hybridization, were 87 ± 5% and 13 ± 5% for Bacillus and Clostridium , respectively. Conclusions: An extreme thermophilic, strict anaerobic, mixed microbial culture with H2-producing potential was enriched from digested household wastes. Significance and Impact of the Study: This study provided a culture with a potential to be applied in reactor systems for extreme thermophilic H2 production from complex organic wastes. |
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Keywords: | extreme thermophilic household wastes hydrogen |
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