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Rapid procedure for the isolation of polyfunctional amino acids in elastin
Authors:R A Green  J A Foster  L B Sandberg
Institution:1. Department of Pathology, University of Utah, Salt Lake City, Utah 84112 USA;2. Department of Surgery, University of Utah, Salt Lake City, Utah 84112 USA
Abstract:A rapid technique for the isolation of the elastin crosslinks, desmosine, isodesmosine, and lysinonorleucine, is reported. Elastin hydrolysates were fractionated by ion-exchange chromatography using volatile buffers. 95% of the desmosine and 86% of the lysinonorleucine were recovered.
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