Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid,salicylic acid,and their related compounds |
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Authors: | Hideyuki Matsuura Arata Aoi Chizuru Satou Mino Nakaya Chikara Masuta Kensuke Nabeta |
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Institution: | (1) Laboratory of Bioorganic Chemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan;(2) Laboratory of Cell Biology and Manipulation, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan |
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Abstract: | Jasmonic acid (JA) and salicylic acid (SA) are plant hormones involved in plant growth and development. Recent studies demonstrated
that presence of a complex interplay between JA and SA signaling pathways to response to pathogenesis attack and biotic stresses.
To our best knowledge, no method has existed for simultaneous analyses of JA, SA, and their related compounds. Especially,
the glucosides are thought to be the storages or the inactivated compounds, but their contribution should be considered for
elucidating the amount of the aglycons. It is also valuable for measuring the endogenous amount of phenylalanine, cinnamic
acid, and benzoic acid that are the biosynthetic intermediates of SA due to the existence of isochorismate pathway to synthesize
SA. We established this method using deuterium labeled compounds as internal standards. This is the first report of simultaneous
analysis of endogenous JA, SA, and their related compounds. Measuring the endogenous JA, SA, and their related compounds that
had been accumulated in tobacco plants proved the practicality of the newly developed method. It was demonstrated that accumulation
of JA, SA and their related compounds were induced in both case of TMV infection and abiotic stresses. |
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Keywords: | Jasmonic acid Salicylic acid Pathogenesis attack |
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