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Sex different effect of antibiotic and probiotic treatment on intestinal microbiota composition in chemically induced liver injury rats |
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| Institution: | 1. General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China;2. Research and Development Center, Guangdong Meilikang Bio-Sciences Ltd., Foshan 528200, China;3. Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Medical University, Dongguan 523808, China;4. Department of Neonatal Surgery, Guangdong Women and Children Hospital, Guangzhou 511400, China;5. State Key Laboratory of Organ Failure Research, Southern Medical University, Guangzhou 510515, China;6. School of Life Sciences, Qilu Normal University, Jinan 250200, China;7. Department of Organ Transplantation, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 511447, China |
| Abstract: | Differences in the gut microbiota and metabolic processes between males and females may explain differences in the risk of liver injury; however, the sex-specific effects of antibiotics and probiotics on these relationships are not clear. We evaluated differences in the gut microbiota and the risk of liver injury between male and female rats after the oral administration of antibiotics or probiotics followed by a period of diethylnitrosamine treatment to chemically induce liver injuryusing high-throughput sequencing of fecal microbiota combined with histological analyses of liver and colon tissues. Our results suggest that the ratio of gram-positive to gram-negative bacteria in kanamycin-treated rats was significantly higher than that of other groups, and this difference persisted for the duration of the experiment. Antibiotics significantly changed the composition of the gut microbiota of experimental rats. Clindamycin caused more diethylnitrosamine-induced damage to livers of male rats. Probiotics did not influencethe gut microbiota; however, they hadprotective effects against liver injury induced by diethylnitrosamine, especially in female rats. These results strengthen our understanding of sex differences in the indirect effects of antibiotics or probiotics on metabolism and liver injury in hosts via the gut microbiota. |
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