Study of vascular endothelial cell morphology during hyperthermia |
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| Affiliation: | 1. Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA;2. School of Life Sciences and Technology, Shanghai Jiao Tong University, 1954 Hua Shan Road, Shanghai 200030, PR China;3. School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907, USA;1. Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino, IRCCS, Milan, Italy;2. Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy;3. Department of Cardiovascular Surgery, Centro Cardiologico Monzino, IRCCS, Milan, Italy;4. Cardiology Department, Azienda Ospedaliera San Gerardo, Monza, Italy;1. Dipartimento di Medicina Interna e Specialistica, Sezione di Reumatologia, Università degli Studi di Palermo, Italy;2. Dipartimento di Biopatologia e Biotecnologie Mediche, Università degli Studi di Palermo, Italy;3. Dipartimento di Biotecnologie e Scienza Clinica Applicata, Divisione di Reumatologia, Scuola di Medicina, Università degli Studi dell''Aquila, Italy;1. Department of Medical Oncology, Mayo Clinic, Rochester, MN;2. Department of Health Sciences Research, Mayo Clinic, Rochester, MN;3. Institute for Biotechnology and Life Science Technologies, Cornell University, Ithaca, NY;4. Proteomcis Core, Mayo Clinic, Rochester, MN;5. Department of Medicine, University of Rochester, NY;6. Clinical Biostatistics, Janssen Pharmaceuticals, Raritan, NJ;1. Enhanced Oil Recovery Institute, China University of Petroleum, Beijing 102249, China;2. Laboratory of Basic Theory for Application of EOR in Low Permeable Oil Fields, CNPC Key Laboratory of EOR, China University of Petroleum, Beijing 102249, China;3. MOE Key Laboratory of Petroleum Engineering, China University of Petroleum, Beijing 102249, China;4. School of Mining and Petroleum, University of Alberta, Edmonton, Alberta, Canada T6G 2W2;1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China;2. University of Chinese Academy of Science, Beijing 10039, PR China;3. Chemistry and Life Science School, Advanced Institute of Materials Science, Changchun University of Technology, 2055 Yanan Street, Changchun, Jilin 130012, PR China;4. Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6 Ontario, Canada;1. Department of Oral and Maxillofacial-Head & Neck Oncology and Faculty of Oral and Maxillofacial Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China;2. Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai 200011, China |
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| Abstract: | In this paper, human umbilical vein endothelial cells (HUVECs) cultured in vitro were used to observe the hyperthermia effect on the dynamic change of the endothelial cell shape and cytoskeletons. A tumor environment was simulated using the ECM625 gel. Hollow tube-like structures were formed of endothelial cells and their focal adhesions were found fewer than those cultured on gelatin. Intercellular gap size increased significantly during hyperthermia. Results showed that cells in the G0/G1 phase or with fewer nucleoli were thermally less sensitive. This suggests that the efficacy of tumor anti-angiogenesis therapy may be promoted by arresting the endothelial cells in the S/G2 phase and applying hyperthermia simultaneously. |
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