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四种园蛛同工酶的比较研究
引用本文:王晓霞,彭宇,刘凤想,黄静,陈婧. 四种园蛛同工酶的比较研究[J]. 蛛形学报, 2003, 12(1): 38-41
作者姓名:王晓霞  彭宇  刘凤想  黄静  陈婧
作者单位:1. 湖北大学生命科学学院,武汉,430062
2. 南京大学数学系,南京,210093
基金项目:国家自然科学基金(30270895),湖北省教育厅基金(2001B01005)
摘    要:本文采用聚丙烯酰胺凝胶电泳的方法,对叶斑园蛛、角类肥蛛(黑、白2种体色)、大腹园蛛、黑斑园蛛4种园蛛科蜘蛛的酯酶(EST)同工酶、乳酸脱氢酶(LDH)同工酶、超氧化物歧化酶(SOD)同工酶进行了比较研究。结果表明,4种园蛛的EST、LDH具有明显的种族特异性,而SOD在种间基本没有差别。不同种蜘蛛都有各自的EST、LDH同工酶谱型,可以用它们作为识别物种的附加指标。

关 键 词:园蛛 同工酶 叶斑园蛛 角类肥蛛 大腹园蛛 黑斑园蛛 酯酶 乳酸脱氢酶 超氧化物歧化酶
修稿时间:2002-06-28

Studies on Isozyme of Four Species of the Genus Araneus (Araneae: Araneidae)
WANG Xiao-Xia PENG Yu LIU Feng-Xiang HUANG Jing CHEN Jing. Studies on Isozyme of Four Species of the Genus Araneus (Araneae: Araneidae)[J]. Acta Arachnologica Sinica, 2003, 12(1): 38-41
Authors:WANG Xiao-Xia PENG Yu LIU Feng-Xiang HUANG Jing CHEN Jing
Abstract:Polyacrylamide gel electrophoresis had been used to study esterase(EST) isozyme, lactate dehydrogenase (LDH) isozyme, superoxide dismutase (SOD) isozyme variations in four species of araneidae, Araneus sia Strand, A.cornutus, A. ventricosus and A.mitificus. The results showed that EST isozyme and LDH isozyme were obvious differences between the species, but there were no differences between the four species in SOD isozyme. Different kinds of spiders had different EST isozyme and LDH isozyme patterns, which could be used to take as distinguishing species' appended targets.
Keywords:Araneidae  esterase isozyme  lactate dehydrogenase isozyme  superoxide dismutase isozyme  polyacrylamide gel electrophoresis  
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