A soluble CO- and NO-bindingc-type cytochrome inNeisseria meningitidis |
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Authors: | E K C Yu I W DeVoe J E Gilchrist |
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Institution: | (1) Department of Microbiology and Immunology, McGill University, H3A 2B4 Montreal, Quebec, Canada |
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Abstract: | Group BNeisseria meningitidis (SDIC) was grown aerobically in Mueller-Hinton medium to stationary phase and then broken either by sonic oscillation or
high pressure extrusion from a French pressure cell. The particulate fraction (345,000×g, 3 h) contained cytochromesc, b, o, and evidence of ana-type. The supernatant fraction contained a solublec-type cytochrome (c
549). The pyridine hemochrome derivatives of acid-acetone-extracted supernatant fractions were shown to be free of other cytochrome
types. The solublec cytochrome increased during the growth cycle of the organism, reaching a maximum after cells entered stationary phase, whereas
the cellular level of other cytochromes from the particulate fraction started to decline as cells progressed from log into
stationary phase. Osmotic shock, as well as other treatments, failed to selectively release the solublec cytochrome from the cell. When untreated, thec
549 in the supernatant fraction remained in a reduced form. While it was not air oxidizable, it was readily oxidizable with K3Fe(CN)6. In the reduced form, thec
549 bound either CO or NO, but in the oxidized form, only NO was bound. The results obtained from this study indicate that the
solublec
549 is probably best classified as a cytochromec′. |
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