Polyamines and thiols in the cytoprotective effect of L-cysteine and L-methionine on carbon tetrachloride-induced hepatotoxicity |
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Authors: | W Chen D O Kennedy A Kojima I Matsui-Yuasa |
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Institution: | (1) Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan, JP |
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Abstract: | Summary. The relationship between cellular glutathione (GSH), protein-SH levels, and lactate dehydrogenase (LDH), with respect to
the effect of polyamines on the cytoprotective ability of L-cysteine and L-methionine, the most important components in the
sulfur amino acid metabolic pathway, in carbon tetrachloride (CCl4)-induced toxicity in isolated rat hepatocytes was studied. CCl4 induced a LDH release and decreased cellular thiols and polyamines levels but treatment with L-cysteine and L-methionine
reversed these decreases. Treating with methylglyoxal bis-(guanylhydrazone), MGBG, an irreversible inhibitor of S-adenosylmethionine
decarboxylase, which is a key enzyme in spermidine and spermine biosynthesis, and therefore used to deplete cellular polyamines,
prevented the protective effect of L-cysteine and L-methionine, but the addition of exogenous polyamines inhibited the influence
of MGBG. These results suggest that the cytoprotective effect of L-cysteine and L-methionine in CCl4-induced toxicity were via maintenance of cellular polyamines, GSH and protein-SH concentrations and prevention of LDH leakage.
Received September 1, 1999, Accepted January 11, 2000 |
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Keywords: | : Amino acids – L-Cysteine – L-Methionine – Polyamines – Glutathione – Protein sulfhydryl – Hepatocytes |
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