Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus |
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Authors: | Yamashita T Iida A Morikawa H |
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Institution: | Department of Biological Sciences, Faculty of Science, Hiroshima University, Hiroshima 730, Japan. |
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Abstract: | Plasmid DNA harboring the β-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus. |
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