Transmembrane 4 L six family member 5 (TM4SF5) enhances migration and invasion of hepatocytes for effective metastasis |
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| Authors: | Sin‐Ae Lee Tae You Kim Tae Kyoung Kwak Hyeonjung Kim Semi Kim Hyo Jeong Lee Sung‐Hoon Kim Ki Hun Park Hyun Jeong Kim Moonjae Cho Jung Weon Lee |
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| Institution: | 1. Cancer Research Institute, Seoul National University, Seoul 110‐799, Korea;2. Department of Molecular & Clinical Oncology, College of Medicine, Seoul National University, Seoul 110‐799, Korea;3. Department of Tumor Biology, College of Medicine, Seoul National University, Seoul 110‐799, Korea;4. KRIBB, Taejon 305‐333, Korea;5. CPMDRC, College of Oriental Medicine, Kyunghee University, Seoul 131‐701, Korea;6. Division of Applied Life Science, EB‐NCRC, Gyeongsang National University, Jinju 660‐701, Korea;7. Department of Dental Anesthesiology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110‐799, Korea;8. Department of Medicine, Cheju National University, Jeju 690‐756, Korea;9. Department of Pharmacy, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Cell Dynamics Research Center, Seoul National University, Seoul 151‐742, Korea |
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| Abstract: | Overexpression of transmembrane 4 L six family member 5 (TM4SF5), a four‐transmembrane L6 family member, causes aberrant cell proliferation and angiogenesis, but the roles of TM4SF5 in migration, invasion, and tumor metastasis remain unknown. Using in vitro hepatocarcinoma cells that ectopically or endogenously express TM4SF5 and in vivo mouse systems, roles of TM4SF5 in metastatic potentials were examined. We found that TM4SF5 expression facilitated migration, invadopodia formation, MMP activation, invasion, and eventually lung metastasis in nude mice, but suppression of TM4SF5 with its shRNA blocked the effects. Altogether, TM4SF5‐mediated migration and invasion suggest that TM4SF5 may be therapeutically targeted to deal with TM4SF5‐mediated hepatocellular cancers. J. Cell. Biochem. 111: 59–66, 2010. © 2010 Wiley‐Liss, Inc. |
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| Keywords: | invadopodia migration invasion TM4SF5 MMP metastasis |
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