Expression of a novel tropomyosin isoform in axolotl heart and skeletal muscle |
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| Authors: | Anish Thomas Sudarsan Rajan Harold L. Thurston Sreeharsha N. Masineni Preeti Dube Abhishek Bose Vasundhara Muthu Syamalima Dube David F. Wieczorek Bernard J. Poiesz Dipak K. Dube |
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| Affiliation: | 1. Department of Medicine, SUNY Upstate Medical University, Syracuse, New York;2. Departments of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio;3. Departments of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio |
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| Abstract: | TPM1κ is an alternatively spliced isoform of the TPM1 gene whose specific role in cardiac development and disease is yet to be elucidated. Although mRNA studies have shown TPM1κ expression in axolotl heart and skeletal muscle, it has not been quantified. Also the presence of TPM1κ protein in axolotl heart and skeletal muscle has not been demonstrated. In this study, we quantified TPM1κ mRNA expression in axolotl heart and skeletal muscle. Using a newly developed TPM1κ specific antibody, we demonstrated the expression and incorporation of TPM1κ protein in myofibrils of axolotl heart and skeletal muscle. The results support the potential role of TPM1κ in myofibrillogenesis and sarcomeric function. J. Cell. Biochem. 110: 875–881, 2010. © 2010 Wiley‐Liss, Inc. |
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