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Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity
Authors:Emma Holder  Barbara Stevenson  Raymond Farley  Tom Hilliard  Theresa Wodehouse  Lucinda Somerton  Mia Larsen  Jean O'Donoghue  Rebecca L. Coles  Ronald K. Scheule  Seng H. Cheng  Deborah R. Gill  Stephen C. Hyde  Uta Griesenbach  Eric W. F. W. Alton  David J. Porteous  A. Christopher Boyd
Affiliation:1. Medical Genetics Section, Molecular Medicine Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK;2. UK Cystic Fibrosis Gene Therapy Consortium, London, Edinburgh, Oxford, UK;3. Department of Gene Therapy, National Heart and Lung Institute, Imperial College, London, UK;4. Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, UK;5. Genzyme Corporation, Framingham, MA, USA
Abstract:
Keywords:CFTR  gene transfer  laser microdissection  nasal brushing  pronase digestion  respiratory epithelium
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