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The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level
Authors:Dominic J Glover  Denisse L Leyton  Gregory W Moseley  Prof David A Jans
Institution:1. Nuclear Signalling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Australia;2. Bacterial Pathogenesis & Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Edgbaston B15 2TT, United Kingdom;3. ARC Centre of Excellence for Biotechnology and Development, Canberra, Australia
Abstract:
Keywords:non‐viral vector  nuclear localisation  gene expression  plasmid DNA  nuclei extraction  flow cytometry
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