Adiponectin increases BMP‐2 expression in osteoblasts via AdipoR receptor signaling pathway |
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| Authors: | Chun‐Yin Huang Chun‐Yi Lee Meng‐Yi Chen Hsiao‐Chi Tsai Horng‐Chaung Hsu Chih‐Hsin Tang |
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| Affiliation: | 1. Department of Orthopaedic Surgery, China Medical University Beigang Hospital, Yun‐Lin County, Taiwan;2. Department of Pharmacology, China Medical University, Taichung, Taiwan;3. Department of Orthopaedic Surgery, China Medical University Hospital, Taichung, Taiwan;4. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan |
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| Abstract: | Adiponectin is a protein hormone secreted predominantly by differentiated adipocytes and involved in energy homeostasis. Bone morphogenetic protein (BMP) plays important roles in osteoblastic differentiation and bone formation. However, the effects of adiponectin on BMPs expression in cultured osteoblasts are largely unknown. Here we found that adiponectin increased mRNA expression of BMP‐2 but not other BMPs in cultured osteoblastic cells. Stimulation of osteoblasts with adiponectin also increased protein levels of BMP‐2 by Western blot and ELISA assay. Adiponectin‐mediated BMP‐2 expression was attenuated by 5′‐AMP‐activated protein kinase (AMPK) small interference RNA and AMPK inhibitor (araA and compound C). Activations of p38 and NF‐κB pathways after adiponectin treatment were demonstrated, and adiponectin‐induced expression of BMP‐2 was inhibited by the specific inhibitor and mutant of p38 and NF‐κB cascades. Taken together, our results provide evidence that adiponectin enhances BMP‐2 expression in osteoblastic cells, and AdipoR1 receptor, AMPK, p38 and NF‐κB signaling pathways may be involved in increasing BMP‐2 expression by adiponectin. J. Cell. Physiol. 224: 475–483, 2010. © 2010 Wiley‐Liss, Inc. |
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