Localization of chromosome breakpoints induced by DNase I in Chinese hamster ovary (CHO) cells |
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| Authors: | Gustavo A Folle Enrique Boccardo Günter Obe |
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| Institution: | (1) División de Citogenética Humana y Microscopía Cuantitativa, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, C.P. 11600, Montevideo, Uruguay, UY;(2) Department of Genetics, University of Essen, P.O. Box 45037, D-45117 Essen, Germany, DE |
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| Abstract: | DNase I was electroporated into S-phase CHO cells and induced chromosome breakpoints were localized in G-banded metaphases.
More than 75% of breakpoints mapped to Giemsa-light bands, 18% to Giemsa-dark bands and about 7% to band junctions. Chromosome
breakpoint clusters produced by DNase I colocalized with chromosome breakpoints induced by the restriction endonucleases AluI and BamHI in the G1- and S-phases of the cell cycle in CHO cells. Digestion of metaphase spreads with AluI, BamHI and DNase I produced G-bands, indicating that G-light bands are more sensitive to endonuclease action. The possible role
of nuclease-sensitive sites in active chromatin as selective targets for the induction of chromosome breakpoints by these
endonucleases is discussed.
Received: 15 January 1997; in revised form: 21 July 1997 / Accepted: 23 July 1997 |
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