Immobilization of trypsin on sand: Mode of binding |
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Authors: | R Puvanakrishnan S M Bose B R Reddi |
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Institution: | (1) Biochemistry Laboratory, Central Leather Research Institute, 600020 Madras;(2) Department of Applied Geology, University of Madras, A. C. College of Technology Campus, 600 025 Madras |
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Abstract: | Acid-washed and heat-treated river sand was separated into different fractions by geochemical methods and immobilization of
trypsin was carried out on the separated fractions using 3-aminopropyltriethoxysilane and glutaraldehyde. Scanning Electron
Micrographs of the purified fraction (Sp. gr >2.5 and <2.8) of magnetically non-susceptible sand and quartz showed that the
enzyme could be fixed on the supports. Malonic acid (16.3 nmol and 16.7nmol per g) appeared to be bound to alkylamine purified
fraction of magnetically non-susceptible sand and alkylamine quartz, respectively. Studies on the effect of 6 M guanidine.HCl
on immobilized trypsin demonstrated that immobilized trypsin had considerable stability against denaturation. The results
obtained indicated that magnetically non-susceptible sand was found to be nearly as good as quartz for trypsin immobilization
and that trypsin was covalently coupled to sand via 3-aminopropyltriethoxysilane and glutaraldehyde. |
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Keywords: | Sand 3-aminopropyltriethoxysilane trypsin quartz scanning electron microscopy |
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