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Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration
Authors:Jiazhang Lian  Run Jin  Huimin Zhao
Institution:1. Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana‐Champaign, Urbana 61801, Illinois;2. School of Molecular and Cellular Biology, University of Illinois at Urbana‐Champaign, Urbana, Illinois;3. (217) 333‐2631(217) 333‐5052;4. Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana‐Champaign, Urbana, Illinois
Abstract:
Keywords:plasmid copy number  pathway optimization  multiplex genome integration  CRISPR/Cas9  synthetic biology
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