Induction of a proteinase by heat-shock in yeast |
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Authors: | Thomas Gross Bernd Schulz-Harder |
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Affiliation: | Freie Universität Berlin, Institut für Biochemie und Molekularbiologie, Ehrenbergstrasse 26–28, D-1000 Berlin 33, F.R.G. |
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Abstract: | Abstract In Saccharomyces cerevisiae heat-shock induces an increase in proteinase activity. The induction is probably due to newly synthesized enzyme molecules, since the increase in proteinase activity can be inhibited by cycloheximide. Degradation of endogenous proteins is enhanced by EDTA, while the azocasein assay is not affected by MnCl2, MgCl2, or EDTA. The proteinase has a pH optimum of 8, and phenylmethylsulfonyl fluoride (PMSF) as well as chymostatin are strong inhibitors. We infer that the induced proteinase is probably identical with proteinase B of yeast. |
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Keywords: | Proteinase activity heat-shock Saccharomyces cerevisiae |
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