Changes in the binding of copper in the plasma of molybdenum supplemented rats |
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Authors: | Hubertus Nederbragt Cornelis J.A. van den Hamer |
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Affiliation: | Zootechnical Institute, Faculty of Veterinary Sciences, State University Utrecht, The Netherlands;Interuniversity Reactor Institute, Delft, The Netherlands |
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Abstract: | After incubating plasma of Mo-supplemented rats (Mo-plasma) with 64Cu only part of it could be removed by dialysis against EDTA or histidine or by treatment with dithiocarbamate; this nondialyzable Cu was shown to be bound to albumin. The maximal amount of 64Cu bound this way equaled the Mo-induced increase in total plasma Cu. After addition of stable Cu dialysis of Mo-plasma against a histidine solution showed that no extra Cu became tightly bound, suggesting that the 64Cu binding was due to an exchange between added 64Cu and stable Cu already present. Incubating Mo-plasma with Hg compounds prevented 64Cu binding and released stable Cu, indicating that Cu in Mo-plasma was sulfhydryl bound. Part of the Mo in Mo-plasma was freely dialyzable. The remaining part was shown to be SH bound as well. The estimated atomic ratio of SH-bound Cu and Mo was unity. Molybdenum increased the number of SH groups in plasma, and for each Cu atom at least one SH group was calculated to be present. |
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Keywords: | Address reprint requests to Dr. H. Nederbragt Department of General Pathology Faculty of Veterinary Sciences State University Utrecht Bilstraat 172 3572 BP Utrecht The Netherlands |
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