Large scale fermentation and alkaloid production of cell suspension cultures of Catharanthus roseus |
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Authors: | Otmar Schiel Jochen Berlin |
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Affiliation: | (1) GBF-Gesellschaft f. Biotechnologische Forschung m.b.H., Mascheroder Weg 1, D-3300 Braunschweig, BRD |
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Abstract: | Cell cultures of Catharanthus roseus were scaled up to volumes of 50001 using conventional reactors equipped with flat-blade impellers. The behavior of the fermenter grown cells was compared with corresponding shake flask experiments with respect to growth and indole alkaloid inducibility and production. The limits and problems of transferring shake flask experiments of culture systems such as Catharanthus, in which alkaloid production depends greatly upon the physiological state of the cells, to large scale multistage processes is discussed. |
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Keywords: | Catharanthus roseus suspension cultures indole alkaloids production fermentation |
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