Cloning and characterization ofMannheimia succiniciproducens MBEL55E phosphoenolpyruvate carboxykinase (pckA) gene |
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| Authors: | Pyung Cheon Lee Sang Yup Lee Soon Ho Hong Ho Nam Chang |
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| Affiliation: | (1) Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 305-701 Daejeon, Korea;(2) Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 305-701 Daejeon, Korea |
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| Abstract: | ApckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacteriumMannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of theM. succiniciproducens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKs. |
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| Keywords: | phosphoenolpyruvate carboxykinase pckA Mannheimia succiniciproducens MBEL55E |
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