Cloning and Characterization of Rat Cellular Nucleic Acid Binding Protein (CNBP) cDNA |
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| Authors: | Yasuda Jun; Mashiyama Shoji; Makino Reiko; Ohyama Shigekazu; Sekiya Takao; Hayashi Kenshi |
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| Institution: | 1Oncogene Division, National Cancer Center Research Institute 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan
2Division of Genome Analysis, Institute of Genetic Information, Kyushu University Maidashi 3-1-1, Higashi-ku, Fukuoka 812, Japan |
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| Abstract: | We cloned and sequenced the cDNAs which code for rat cellularnucleic acid binding protein (CNBP). In-frame insertion/deletiondifferences were found among the clones at two sites in theopen reading frame, suggesting alternative splicing of the messageor the presence of multiple genes which code for this protein.The deduced amino acid sequence revealed that one rat CNBP sequencewas completely identical to its human counterpart. This strikingconservation, together with the fact that homologous genes havebeen found in various organisms including Schizosaccharomycespombe, suggests that CNBP plays a basic biological role in eukaryoticcells. The recombinant GST-CNBP fusion protein produced in Escherichiacoli bound to a G-rich single-stranded RNA and DNA in a sequence-specificmanner. |
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| Keywords: | CCHC box zinc finger GST-CNBP fusion protein RNA binding |
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