Inhibition of lactate dehydrogenase in cultured SIRC cells by cigarette smoke |
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Authors: | Norman Fleming Heinrich Walt Gonzague S. Kistler |
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Affiliation: | (1) Division of Cell Biology, Institute of Anatomy, University of Zürich, Gloriastraße 19, CH-8006 Zürich, Switzerland;(2) Present address: Department of Pathology, The Research Institute, The Hospital for Sick Children, 555, University Avenue, M5G 1x8 Toronto, Ontario, Canada |
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Abstract: | Summary SIRC cell monolayer cultures were exposed to whole smoke from a mid tar and nicotine level research cigarette (ASFC, 72 puffs), or from a high tar and nicotine level reference cigarette (Kentucky 2R1, 48 puffs) over a period of 65 days. The activity and distribution of lactate dehydrogenase (LDH) in the cells were investigated, and the electrophoretic characteristics of its isozymes studied. Cell morphology was examined by light microscopy and by transmission- and scanning electron microscopy.LDH activity was reduced by exposure to smoke from both cigarette types, the greater inhibitory effect being produced by that of the Kentucky cigarette. In addition, cells exposed to this high tar and nicotine smoke displayed intramitochondrial granules which were larger and more numerous than those found in cells exposed to the mid tar and nicotine smoke, or in the control cells. It is speculated that cation accumulation in the mitochondria may be involved in the observed inhibition of LDH activity.Supported by a research grant from the ASFC (Association Suisse des Fabricants de Cigarettes), Switzerland |
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