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Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding
Authors:Verdecia Mark A  Larkin Robert M  Ferrer Jean-Luc  Riek Roland  Chory Joanne  Noel Joseph P
Institution:1 Chemical Biology and Proteomics Laboratory, Salk Institute for Biological Studies La Jolla, California United States of America;2 Howard Hughes Medical Institute, Plant Molecular and Cellular Biology Laboratory Salk Institute for Biological Studies, La Jolla, California United States of America;3 The Institut de Biologie Structurale Grenoble France;University of North Carolina United States of America
Abstract:In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activity of Mg-chelatase, the enzyme that synthesizes Mg-Proto. GUN4 may also play a role in both photoprotection and the cellular shuttling of tetrapyrroles. Here we report a 1.78-Å resolution crystal structure of Synechocystis GUN4, in which the porphyrin-binding domain adopts a unique three dimensional fold with a “cupped hand” shape. Biophysical and biochemical analyses revealed the specific site of interaction between GUN4 and Mg-Proto and the energetic determinants for the GUN4 • Mg-Proto interaction. Our data support a novel protective function for GUN4 in tetrapyrrole trafficking. The combined structural and energetic analyses presented herein form the physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity, as well as in Mg-Proto retrograde signaling.
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